Kedu ihe bụ tubes na-anakọta ọbara EDTA yana otu esi eji ha?

Na nyocha ahụike na nyocha ụlọ ọgwụ na ọgwụgwọ,Ọbara mkpokọta EDTA, dị ka isi ihe oriri maka nchịkọta ọbara, na-ekere òkè dị mkpa n'ịkwado iguzosi ike n'ezi ihe nke sample na izi ezi nke ule. N'isiokwu a, anyị ga-enyocha nke ọma "onye nlekọta a na-adịghị ahụ anya" na ngalaba ahụike site na akụkụ nke nkọwa, nhazi agba, ụkpụrụ mgbochi mgbochi, ebumnuche ule na ọkọlọtọ ojiji.

Gịnị bụtube mkpokọta ọbara EDTA?

Ọbara nchịkọta ọbara EDTA bụ ụdị ọkpọkọ mkpokọ ọbara nke nwere Ethylene Diamine Tetraacetic Acid ma ọ bụ nnu ya, nke a na-ejikarị maka nchịkọta ọbara na ọgwụgwọ ọgwụ mgbochi. EDTA nwere ike igbochi mmeghachi omume coagulation cascade site n'ịchịkọta ion calcium n'ime ọbara, iji mee ka ọbara dị na mmiri mmiri ruo ogologo oge, ma nyekwa ihe nlele kwụsiri ike maka nyocha nke usoro ọbara na usoro ihe ọmụmụ ihe. Ọ na-enye ihe nlele kwụsiri ike maka usoro ọbara, usoro ihe ọmụmụ ihe na nyocha ndị ọzọ.

Dị ka akụkụ dị mkpa nkeọgwụ consumables, EDTA ọbara nchịkọta tubes kwesịrị ịgbaso ụkpụrụ mba nke "Ngwakọta ihe nchịkọta ọbara nke otu-eji otu" (dịka GB / T 19489-2008) iji hụ na arụmọrụ nke sterility, ndị na-abụghị pyrogenic na ndị na-abụghị cytotoxicity.

Agba dị iche iche nke tubes nchịkọta ọbara EDTA

Dị ka ụkpụrụ mba ụwa si dị (dịka ntuziaka CLSI H3-A6), a na-ekpuchi tubes nchịkọta ọbara EDTA na-acha odo odo (EDTA-K2/K3) ma ọ bụ acha anụnụ anụnụ (sodium citrate agwakọta na EDTA) iji mata ọdịiche dị na ojiji:

Mara: Ụfọdụ ụdị nwere ike itinye koodu na agba ndị ọzọ, lelee ntuziaka tupu ejiri ya.

Usoro mgbochi mgbochi nke tubes nchịkọta ọbara EDTA

EDTA site na molecular carboxyl group (-COOH) na calcium ions n'ime ọbara (Ca²⁺) jikọtara ọnụ iji mepụta chelate kwụsiri ike, si otú a na-egbochi ọrụ plasminogen, na-egbochi usoro coagulation nke fibrinogen n'ime fibrin. Anticoagulation a nwere njirimara ndị a:

1. mmalite ngwa ngwa: enwere ike ịmecha ọgwụ mgbochi n'ime nkeji 1-2 mgbe nchịkọta ọbara gasịrị;

2. elu kwụsie ike: samples nwere ike ịchekwa maka ihe karịrị 48 awa (refrigerated nwere ike ịgbatị ruo 72 awa);

3. Ngwa dịgasị iche iche: adabara maka ọtụtụ ule hematology, ma ọ bụghị maka coagulation ma ọ bụ ule ọrụ platelet (a chọrọ tubes sodium citrate).

Ihe nyocha isi nke tube mkpokọta ọbara EDTA

1. nyocha ọbara oge niile: ọnụ ọgụgụ sel ọbara ọcha, mkpụrụ ndụ ọbara uhie uhie, mkpokọta hemoglobin, wdg;

2. njirimara nke otu ọbara na cross-matching: ABO blood group, Rh factor detection;

3. nchoputa nke mkpụrụ ndụ: nyocha mkpụrụ ndụ ihe nketa, mkpebi siri ike nke nje virus (dịka HIV, HBV);

4. glycated haemoglobin (HbA1c): ogologo oge nlekota glucose ọbara maka ọrịa shuga mellitus;

5. Nyocha ọbara parasaiti: Plasmodium, nchọpụta microfilariae.

Iji ụkpụrụ na ịkpachara anya

1. Usoro nchịkọta:

Mgbe emechara akpụkpọ ahụ, rụọ ọrụ dịka ọkọlọtọ nke nchịkọta ọbara venous si dị;

Ozugbo nchịkọta, tụgharịa tube nchịkọta ọbara ugboro 5-8 iji hụ na ọgwụ mgbochi ahụ jikọtara ya na ọbara;

Zere ịma jijiji ime ihe ike (iji gbochie hemolysis).

2. Nchekwa na ụgbọ njem:

Na-echekwa na ụlọ okpomọkụ (15-25 Celsius C), zere okpomọkụ ma ọ bụ kefriza;

Debe kwụ ọtọ n'oge njem iji gbochie ịtọpụ okpu tube.

3. contraindications ndapụta:

A na-achọ tubes sodium citrate maka Coagulation IV (PT, APTT, wdg);

Nnwale ọrụ platelet chọrọ tube sodium citrate.

Otu esi ahọrọ oke mmatube mkpokọta ọbara EDTA?

1. Ikike na asambodo: họrọ ngwaahịa ndị gafere ISO13485 na CE asambodo. 2;

2. Nchekwa ihe: ahụ tube kwesịrị ịbụ nke ọma na enweghị ihe fọdụrụnụ nke plasticizer;

3. Dosing ziri ezi: ọnụ ọgụgụ nke anticoagulant agbakwunyere kwesịrị ịdị na ụkpụrụ mba (dịka ọmụmaatụ EDTA-K2 ntinye nke 1.8 ± 0.15mg / mL);

4. Aha aha: A na-ebute ụzọ maka ụdị ndị a ma ama n'ọhịa nke ihe oriri ọgwụ iji hụ na nkwụsi ike batch.

Mmechi

Dị ka isi otu nkengwaọrụ nchịkọta ọbara, Ọkụ nchịkọta ọbara EDTA na-enwe mmetụta kpọmkwem na izi ezi nke nsonaazụ ule n'ihe gbasara ihe mgbochi ha. Site n'ịhazi usoro iji tubes na-anakọta ọbara dị iche iche na-agbakọta na ijikọta ha na usoro nchịkọta siri ike, ọ nwere ike inye ihe ndabere a pụrụ ịdabere na ya maka nchọpụta ụlọ ọgwụ. N'ọdịnihu, site na mmepe nke ọgwụgwọ ziri ezi, tubes nchịkọta ọbara EDTA ga-arụ ọrụ dị mkpa na nyocha ọbara, usoro mkpụrụ ndụ ihe nketa na mpaghara ndị ọzọ, ma nọgide na-echebe ahụike mmadụ.