Kedu ihe bụ EDTA Blood Collection Tubes, kedu ka esi eji ha?

N'ule ahụike na nchọpụta na ọgwụgwọ ahụike,Ọkpọkọ ọbara EDTADịka ihe ndị dị mkpa maka ịnakọta ọbara, ọ na-arụ ọrụ dị mkpa n'ịhụ na ihe nlele ahụ ziri ezi ma bụrụkwa eziokwu. N'isiokwu a, anyị ga-enyocha "onye nche a na-anaghị ahụ anya" nke ọma n'ọhịa ahụike site na nkọwapụta, nhazi agba, ụkpụrụ mgbochi coagulation, ebumnuche nnwale na ọkọlọtọ ojiji.

Gịnị bụỌdụdọ nchịkọta ọbara EDTA?

Ọkpọkọ ọbara EDTA bụ ụdị ọkpọkọ ọbara vacuum nke nwere Ethylene Diamine Tetraacetic Acid ma ọ bụ nnu ya, nke a na-ejikarị eme ihe maka nchịkọta ihe nlele ọbara na ọgwụgwọ anticoagulants. EDTA nwere ike igbochi mmeghachi omume coagulation cascade site na ịmịpụta ion calcium dị n'ọbara, ka o wee debe ọbara ahụ n'ọnọdụ mmiri mmiri ruo ogologo oge, ma nye ihe nlele kwụsiri ike maka ule usoro ọbara na bayoloji molekul. Ọ na-enye ihe nlele kwụsiri ike maka usoro ọbara, bayoloji molekul na ule ndị ọzọ.

Dịka akụkụ dị mkpa nkeihe oriri ahụike, ọkpọkọ ọbara EDTA kwesịrị ịgbaso ọkọlọtọ mba nke "Igbe nchịkọta ọbara venous nke ejiri otu oge" (dịka ọmụmaatụ GB/T 19489-2008) iji hụ na arụmọrụ ya dị mma, na-abụghị pyrogenic na na-adịghị egbu egbu.

Agba dị iche iche nke ọkpọkọ ọbara EDTA

Dịka ụkpụrụ mba ụwa si dị (dịka ntuziaka CLSI H3-A6), a na-ejikarị acha odo odo (EDTA-K2/K3) ma ọ bụ acha anụnụ anụnụ (sodium citrate agwakọtara ya na EDTA) kpuchie ọkpọkọ ọbara EDTA iji mata ọdịiche dị n'etiti ojiji:

Rịba ama: Enwere ike itinye koodu n'ụfọdụ ụdị ngwaahịa na agba ndị ọzọ, lelee ntuziaka tupu ejiri ya.

Usoro mgbochi coagulation nke ọkpọkọ nchịkọta ọbara EDTA

EDTA site na otu carboxyl molekul ya (-COOH) na calcium ion n'ọbara (Ca²⁺) jikọtara ọnụ iji mepụta chelate kwụsiri ike, si otú a na-egbochi ọrụ nke plasminogen, na-egbochi usoro coagulation nke fibrinogen ka ọ banye na fibrin. Ọgwụ mgbochi coagulation a nwere njirimara ndị a:

1. mmalite ngwa ngwa nke ọrụ: enwere ike imechi ọgwụ mgbochi ọbara n'ime nkeji 1-2 mgbe anakọtachara ọbara;

2. nkwụsi ike dị elu: enwere ike ịchekwa ihe nlele ihe karịrị awa 48 (enwere ike ịgbatị ha na friji ruo awa 72);

3. Ọtụtụ usoro ojiji: ọ dị mma maka ọtụtụ ule ọbara, mana ọ bụghị maka ule coagulation ma ọ bụ platelet function (a chọrọ sodium citrate tubes).

Ihe nnwale isi nke ọkpọkọ nnakọta ọbara EDTA

1. nyocha ọbara nkịtị: ọnụọgụ mkpụrụ ndụ ọbara ọcha, paramita mkpụrụ ndụ ọbara uhie, ntinye hemoglobin, wdg;

2. njirimara otu ọbara na njikọta nke otu ọbara: otu ọbara ABO, nchọpụta Rh factor;

3. nchọpụta mkpụrụ ndụ ihe nketa: nnwale mkpụrụ ndụ ihe nketa, mkpebi ibu nje (dịka ọmụmaatụ HIV, HBV);

4. glycated hemoglobin (HbA1c): nlekota shuga ọbara ogologo oge maka ọrịa shuga mellitus;

5. Nnyocha nje ọbara: Plasmodium, nchọpụta microfilariae.

Ojiji nke ụkpụrụ na ihe ndị a na-eme iji gbochie ihe ize ndụ

1. Usoro nchịkọta:

Mgbe ị sachara akpụkpọ ahụ, jiri usoro nchịkọta ọbara dị mkpa;

Ozugbo e chịkọtara ya, tụgharịa ọkpọkọ ọbara ugboro ise ruo asatọ iji hụ na ihe na-egbochi ọbara agwakọtara ya nke ọma;

Zere ịma jijiji ike (iji gbochie hemolysis).

2. Nchekwa na njem:

Debe ya n'ebe okpomọkụ dị n'ime ụlọ (15-25°C), zere ikpo ọkụ ma ọ bụ oyi;

Debe ya n'ebe kwụ ọtọ n'oge njem iji gbochie mwepụ nke mkpuchi ọkpọkọ ahụ.

3. ọnọdụ ndị na-emegiderịta onwe ha:

A chọrọ ọkpọkọ sodium citrate maka Coagulation IV (PT, APTT, wdg);

Nnwale ọrụ platelet chọrọ sodium citrate tube.

Otu esi ahọrọ àgwà dị eluỌdụdọ nchịkọta ọbara EDTA?

1. Asambodo na asambodo: họrọ ngwaahịa ndị gafere asambodo ISO13485 na CE. 2;

2. Nchekwa ihe: ahụ ọkpọkọ ahụ kwesịrị ịdị mfe nghọta ma ghara inwe ihe fọdụrụ na plasticizer;

3. Usoro ọgwụgwọ ziri ezi: ọnụọgụ ọgwụ mgbochi mkpali agbakwunyere kwesịrị ikwekọ na ọkọlọtọ mba (dịka ọmụmaatụ, ntinye EDTA-K2 nke 1.8±0.15mg/mL);

4. Aha njirimara: A na-enye ndị a ma ama n'ihe gbasara ihe oriri ọgwụ mkpa iji hụ na otu ngwaahịa kwụsiri ike.

Mmechi

Dịka onye otu dị mkpa nkengwaọrụ ịnakọta ọbara, ọkpọkọ ọbara EDTA nwere mmetụta kpọmkwem na izi ezi nke nsonaazụ ule n'ihe gbasara ihe ndị na-egbochi ọbara ha. Site n'ịhazi ojiji nke ọkpọkọ ọbara dị iche iche nwere agba dị iche iche ma jikọta ha na usoro nchịkọta siri ike, ọ nwere ike inye ntọala a pụrụ ịdabere na ya maka nchọpụta ahụike. N'ọdịnihu, site na mmepe nke ọgwụ ziri ezi, ọkpọkọ ọbara EDTA ga-arụ ọrụ dị mkpa karị na nyocha ọbara, usoro mkpụrụ ndụ ihe nketa na mpaghara ndị ọzọ, ma nọgide na-echebe ahụike mmadụ.